Skip to main content
SpringerLink
Log in

Mungbean seed coat water extract inhibits inflammation in LPS-induced acute liver injury mice and LPS-stimulated RAW 246.7 macrophages via the inhibition of TAK1/IκBα/NF-κB

  • Original Article
  • Published:
Journal of Food Science and Technology Aims and scope Submit manuscript

Abstract

Inflammation plays an important role in pathogenesis and progression of many chronic diseases. Although, anti-inflammatory activities of mungbean have been suggested, the underlying mechanism have not been fully understood. The present study aimed to reveal the anti-inflammatory effects of mungbean seed coat water extract (MSWE) in lipopolysaccharide (LPS)-stimulated inflammation in RAW 246.7 macrophages and LPS-induced acute liver injury mice. MSWE pretreatment downregulated the elevated expression of inflammatory markers induced by LPS in the transcriptional and protein level. MSWE inhibited NF-κB activation through the suppression of phosphorylated p65 subunit, IκBα degradation, and transforming growth factor-β-activated kinases 1 (TAK1) phosphorylation in LPS-stimulated RAW 246.7 cells. Vitexin, the major flavonoid in MSWE showed similar effects. In in vivo experiments, we found that oral administration of MSWE downregulated iNOS expression in LPS-induced acute liver injury mice. The mRNA expression of inflammatory markers and macrophage infiltration was also decreased in the livers. Collectively, MSWE exerts anti-inflammatory role, in part possibly through its active compound vitexin, by inhibiting NF-κB activation via inhibition of TAK1 phosphorylation and IκBα degradation. This suggests that MSWE is beneficial to combat various inflammatory diseases.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Çalışkantürk Karataş S, Günay D, Sayar S (2017) In vitro evaluation of whole faba bean and its seed coat as a potential source of functional food components. Food Chem 230:182–188

    Article  Google Scholar 

  • Hamesch K, Borkham-Kamphorst E, Strnad P, Weiskirchen R (2015) Lipopolysaccharide-induced inflammatory liver injury in mice. Lab Anim 49:37–46

    Article  CAS  Google Scholar 

  • Hiratsuka S, Watanabe A, Sakurai Y et al (2008) The S100A8-serum amyloid A3-TLR4 paracrine cascade establishes a pre-metastatic phase. Nat Cell Biol 10:1349–1355

    Article  CAS  Google Scholar 

  • Jang YH, Kang MJ, Choe EO, Shin M, Kim JI (2014) Mung bean coat ameliorates hyperglycemia and the antioxidant status in type 2 diabetic db/db mice. Food Sci Biotechnol 23:247–252

    Article  CAS  Google Scholar 

  • Kang I, Choi S, Ha TJ et al (2015) Effects of Mung Bean (Vigna radiata L.) Ethanol extracts decrease proinflammatory cytokine-induced lipogenesis in the KK-Ay diabese mouse model. J Med Food 18:841–849

    Article  CAS  Google Scholar 

  • Ko WK, Lee SH, Kim SJ et al (2017) Anti-inflammatory effects of ursodeoxycholic acid by lipopolysaccharide-stimulated inflammatory responses in RAW 2647 macrophages. PLoS ONE 12:e0180673. https://doi.org/10.1371/journal.pone.0180673

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lee SJ, Lee JH, Lee HH et al (2011) Effect of mung bean ethanol extract on pro-inflammtory cytokines in LPS stimulated macrophages. Food Sci Biotechnol. https://doi.org/10.1007/s10068-011-0072-z

    Article  Google Scholar 

  • Leon CG, Tory R, Jia J, Sivak O, Wasan KM (2008) Discovery and development of toll-like receptor 4 (TLR4) antagonists: a new paradigm for treating sepsis and other diseases. Pharm Res 25:1751–1761

    Article  CAS  Google Scholar 

  • Li GH, Wan JZ, Le GW, Shi YH (2006) Novel angiotensin I-converting enzyme inhibitory peptides isolated from Alcalase hydrolysate of mung bean protein. J Pept Sci 12:509–514

    Article  CAS  Google Scholar 

  • Li H, Cao D, Yi J, Cao J, Jiang W (2012) Identification of the flavonoids in mungbean (Phaseolus radiatus L.) soup and their antioxidant activities. Food Chem 135:2942–2946

    Article  CAS  Google Scholar 

  • Li AP, Li ZY, Jia JP, Qin XM (2016) Chemical comparison of coat and kernel of mung bean by nuclear magnetic resonance-based metabolic fingerprinting approach. Spectrosc Lett 49:217–224

    Article  CAS  Google Scholar 

  • Liu T, Zhang L, Joo D, Sun SC (2017) NF-κB signaling in inflammation. Signal Transduct Target Ther. https://doi.org/10.1038/sigtrans.2017.23

    Article  PubMed  PubMed Central  Google Scholar 

  • Luo JQ, Cai WX, Wu T, Xu BJ (2016) Phytochemical distribution in hull and cotyledon of adzuki bean (Vigna angularis L.) and mung bean (Vigna radiate L.), and their contribution to antioxidant, anti-inflammatory and anti-diabetic activities. Food Chem 201:350–360

    Article  CAS  Google Scholar 

  • Ma FY, Tesch GH, Ozols E, Xie M, Scheneider MD, Nikolic-Paterson DJ (2011) TGF-beta1-activated kinase-1 regulates inflammation and fibrosis in the obstructed kidney. Am J Physiol Renal Physiol 300:F1410–F1421

    Article  CAS  Google Scholar 

  • Nair RM, Yang RY, Easdown WJ, Thavarajah D, Thavarajah P, Hughes JD, Keatinge JDH (2013) Biofortification of mungbean (Vigna radiata) as a whole food to enhance human health. J Sci Food Agric 93:1805–1813

    Article  CAS  Google Scholar 

  • Neubert M, Ridder DA, Bargiotas P, Akira S, Schwaninger M (2011) Acute inhibition of TAK1 protects against neuronal death in cerebral ischemia. Cell Death Differ 18:1521

    Article  CAS  Google Scholar 

  • Pang HY, Liu G, Liu GT (2009) Compound FLZ inhibits lipopolysaccharide-induced inflammatory effects via down-regulation of the TAK-IKK and TAK-JNK/p38MAPK pathways in RAW264.7 macrophages. Acta Pharmacol Sin 30:209–218

    Article  CAS  Google Scholar 

  • Park SW, Choi J, Kim J et al (2018) Anthocyanins from black soybean seed coat prevent radiation-induced skin fibrosis by downregulating TGF-beta and Smad3 expression. Arch Dermatol Res 310:401–412

    Article  CAS  Google Scholar 

  • Peng X, Zheng Z, Cheng KW et al (2008) Inhibitory effect of mung bean extract and its constituents vitexin and isovitexin on the formation of advanced glycation endproducts. Food Chem 106:475–481

    Article  CAS  Google Scholar 

  • Randhir R, Shetty K (2007) Mung beans processed by solid-state bioconversion improves phenolic content and functionality relevant for diabetes and ulcer management. Innov Food Sci Emerg Technol 8:197–204

    Article  CAS  Google Scholar 

  • Sachithanandan N, Graham KL, Galic S et al (2011) Macrophage deletion of SOCS1 increases sensitivity to LPS and palmitic acid and results in systemic inflammation and hepatic insulin resistance. Diabetes 60:2023–2031

    Article  CAS  Google Scholar 

  • Sakurai H (2012) Targeting of TAK1 in inflammatory disorders and cancer. Trends Pharmacol Sci 33:522–530

    Article  CAS  Google Scholar 

  • Sanada Y, Yamamoto T, Satake R et al (2016) Serum amyloid A3 gene expression in adipocytes is an indicator of the interaction with macrophages. Sci Rep. https://doi.org/10.1038/srep38697

    Article  PubMed  PubMed Central  Google Scholar 

  • Singh B, Singh JP, Shevkani K, Singh N, Kaur A (2017) Bioactive constituents in pulses and their health benefits. J Food Sci Technol 54:858–870

    Article  CAS  Google Scholar 

  • Srivastava R, Burbach BJ, Shimizu Y (2010) NF-kappaB activation in T cells requires discrete control of IkappaB kinase alpha/beta (IKKalpha/beta) phosphorylation and IKKgamma ubiquitination by the ADAP adapter protein. J Biol Chem 285:11100–11105

    Article  CAS  Google Scholar 

  • Takaesu G, Surabhi RM, Park KJ, Ninomiya-Tsuji J, Matsumoto K, Gaynor RB (2003) TAK1 is Critical for IκB Kinase-mediated Activation of the NF-κB Pathway. J Mol Biol 326:105–115

    Article  CAS  Google Scholar 

  • Takeuchi O, Akira S (2010) Pattern recognition receptors and inflammation. Cell 140:805–820

    Article  CAS  Google Scholar 

  • Veres B, Radnai B, Gallyas F et al (2004) Regulation of kinase cascades and transcription factors by a poly(ADP-Ribose) polymerase-1 inhibitor, 4-Hydroxyquinazoline, in lipopolysaccharide-induced inflammation in mice. J Pharmacol Exp Ther 310:247–255

    Article  CAS  Google Scholar 

  • Watanabe H, Inaba Y, Kimura K et al (2016) Dietary mung bean protein reduces hepatic steatosis, fibrosis, and inflammation in male mice with diet-induced, nonalcoholic fatty liver disease. J Nutr 147:52–60

    Article  Google Scholar 

  • Xie CL, Li JL, Xue EX et al (2018) Vitexin alleviates ER-stress-activated apoptosis and the related inflammation in chondrocytes and inhibits the degeneration of cartilage in rats. Food Funct 9:5740–5749

    Article  CAS  Google Scholar 

  • Yanaka N, Koyama TA, Komatsu SI, Nakamura E, Kanda M, Kato N (2005) Vitamin b6 suppresses NF-kappa B activation in LPS-stimulated mouse macrophages. Int J Mol Med 16:1071–1075

    CAS  PubMed  Google Scholar 

  • Yao Y, Chen F, Wang M, Wang J, Ren G (2008) Antidiabetic activity of Mung bean extracts in diabetic KK-Ay mice. J Agric Food Chem 56:8869–8873

    Article  CAS  Google Scholar 

  • Yoshioka Y, Li X, Zhang T et al (2017) Black soybean seed coat polyphenols prevent AAPH-induced oxidative DNA-damage in HepG2 cells. J Clin Biochem Nutr 60:108–114

    Article  CAS  Google Scholar 

  • Zhang XW, Shang PP, Qin F et al (2013) Chemical composition and antioxidative and anti-inflammatory properties of ten commercial mung bean samples. Lwt Food Sci Technol 54:171–178

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by Grant-in-Aid for International Cooperative Research, Graduate School of Integrated Sciences for Life, Hiroshima University, Japan.

Author information

Authors and Affiliations

  1. Department of Food Science and Technology, Faculty of Agro-Industry, Kasetsart University, 50 Ngamwongwan Rd. Ladyao, Chatuchak, Bangkok, Thailand

    Sudathip Sae-tan

  2. Graduate School of Integrated Sciences for Life, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, Hiroshima, Japan

    Thanutchaporn Kumrungsee & Noriyuki Yanaka

Authors
  1. Sudathip Sae-tan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Thanutchaporn Kumrungsee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Noriyuki Yanaka
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Sudathip Sae-tan or Noriyuki Yanaka.

Ethics declarations

Conflict of interest

The other authors have no interests to disclose.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 80 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sae-tan, S., Kumrungsee, T. & Yanaka, N. Mungbean seed coat water extract inhibits inflammation in LPS-induced acute liver injury mice and LPS-stimulated RAW 246.7 macrophages via the inhibition of TAK1/IκBα/NF-κB. J Food Sci Technol 57, 2659–2668 (2020). https://doi.org/10.1007/s13197-020-04302-y

Download citation

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13197-020-04302-y

Keywords

Search

Navigation